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乙型流感病毒抗原診斷試劑盒(膠體金法)
廣州健侖生物科技有限公司
廣州健侖長期供應(yīng)各種流感檢測試劑,包括進(jìn)口和國產(chǎn)的品牌,主要包括日本富士瑞必歐、日本生研、美國BD、美國NovaBios、美國binaxNOW、凱必利、廣州創(chuàng)侖等主流品牌。
乙型流感病毒抗原診斷試劑盒(膠體金法)
我司還提供其它進(jìn)口或國產(chǎn)試劑盒:登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測、食品安全檢測等試劑盒以及日本生研細(xì)菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。
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【公司名稱】 廣州健侖生物科技有限公司
【市場部】 楊永漢
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【騰訊 】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室
一般的sp法步驟啊,具體如下:
1、烤片,68℃,20分鐘,
2、常規(guī)二甲苯脫蠟,梯度酒精脫水;二甲苯I20min?二甲苯II20min?100%酒精10min?100%酒精10min?95%酒精5min?80%酒精5min?70%酒精5min
3、阻斷滅活內(nèi)源性過氧化物酶:3%H2O237℃孵育10min,PBS沖洗3X5min;
4、抗原修復(fù):置0.01M枸櫞酸緩沖液(PH6.0)中用煮沸(95℃,15-20min),自然冷卻20min以上,再用冷水沖洗缸子,加快冷卻至室溫,PBS沖洗3X5min。
5、正常羊血清工作液封閉,37℃10min,傾去勿洗。
6、滴加一抗4℃冰箱孵育過夜,PBS沖洗3X5min(用PBS緩沖液代替一抗作陰性對照);
7、滴加生物素標(biāo)記二抗,37℃孵育30min,PBS沖洗3X5min;
8、滴加辣根過氧化物酶標(biāo)記的鏈霉素卵白素工作液,37℃孵育30min,PBS沖洗3X5min;
9、DAB/H2O2反應(yīng)染色,自來水充分沖洗后,蘇木素復(fù)染,常規(guī)脫水,透明,干燥,封片。
General sp method steps ah, as follows:
1, baked slices, 68 ℃, 20 minutes,
2, normal xylene dewaxing, gradient alcohol dehydration; xylene I20min xylene II20min 100% alcohol 10min 100% alcohol 10min 95% alcohol 5min 80% alcohol 5min 70% alcohol 5min
3, block the inactivation of endogenous peroxidase: 3% H2O237 ℃ 10min incubation, PBS wash 3X5min;
4. Antigen Recover: Set 0.01M citrate buffer (PH6.0) with boiling (95 ℃, 15-20min) for more than 20min, then rinse the cylinder with cold water, cool to room temperature and rinse with PBS for 3X5min.
5, normal sheep serum working fluid closed, 37 ℃ 10min, do not dump.
6, dropping the first antibody incubated overnight at 4 ℃ refrigerator, PBS wash 3X5min (PBS buffer instead of primary antibody as a negative control);
7, dropping biotin labeled secondary antibody, 37 ℃ incubated 30min, PBS wash 3X5min;
8, dropping horseradish peroxidase labeled streptomycin avidin working solution, incubated at 37 ℃ 30min, PBS washed 3X5min;
9, DAB / H2O2 reaction staining, tap water fully washed, hematoxylin counterstain, conventional dehydration, transparent, dry, sealing pieces.