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日本積水流感病毒抗原檢測試劑盒
廣州健侖生物科技有限公司
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日本積水流感病毒抗原檢測試劑盒
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日本積水流感病毒抗原檢測試劑盒
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【公司名稱】 廣州健侖生物科技有限公司
【市場部】 楊永漢
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【騰訊 】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-103室
包埋劑embedding agent
在制作切片或超薄切片時,由于組織是柔軟的,或局部的軟硬不均,這樣制作厚薄均勻的切片是困難的。所以有必要用一定物質(zhì)浸透組織內(nèi)部,整個組織一樣硬化,以利于切成薄片,這種物質(zhì)叫做包埋劑。一股用光學顯微鏡觀察的切片,用石蠟、火棉膠、炭蠟、明膠等作包埋劑;電子顯微鏡則用環(huán)氧樹脂、聚苯乙烯樹脂、異丁烯樹脂及水溶性樹脂。
石蠟制片法是將材料經(jīng)固定、脫水、透明、浸蠟后包埋在石蠟里面進行切片的方法。此法適用范圍,制片手段完備,能將材料切成極薄的片子(可切至2-8微米左右),并能制成連續(xù)的片子,這是其它制片法難以達到的,因此在光學顯微鏡的制片技術上它是zui常用的一種方法。除了一些經(jīng)不住石蠟切片法中所應用的各種藥劑處理的材料不能應用石蠟切片以外,一般的材料都可以用此法制片,但有制作時間較長,操作過程復雜以及材料易發(fā)硬或發(fā)脆等缺點。該法多采用旋轉(zhuǎn)式切片機進行切片。
石蠟制片操作程序如下:
(一)材料的采集與分割
采集材料應根據(jù)制片的目的和要求而定,材料要有代表性,無病蟲害或其它損傷,如要觀察病理解剖,則要取病斑、病癥部位。采下的材料應立即放在標本箱或濕布包起來帶回實驗室進行分割固定(如有條件也可在試驗地采集后進行分割固定)。為使固定液能迅速的滲透材料,材料要進行分割。分割時動作要迅速,以防材料萎蔫。分割塊的大小,宜小不宜大,一般不超過1cm3,分割后立即殺死固定。
Embedding agent
In the production of sliced ??or ultra-thin slices, because the tissue is soft, or local soft and hard uneven, so that the thickness of the slice is difficult to make. So it is necessary to use a certain substance soaked inside the tissue, the entire tissue as hardened to facilitate the cut into thin slices, this material is called embedding agent. An optical microscope observation of the sections with paraffin, collodion, charcoal wax, gelatin and other embedding agents; electron microscopy epoxy resin, polystyrene resin, isobutylene resin and water-soluble resin.
Paraffin preparation method is the material by the fixed, dehydrated, transparent, immersed wax embedded in paraffin inside the method of slicing. The scope of application of this method, the means of production is complete, the material can be cut into very thin films (can be cut to 2-8 microns), and can be made into a continuous film, which is difficult to achieve by other production methods, so in Light microscopy is technically the most commonly used method of making it. In addition to some paraffin sections can not be used in the treatment of various pharmaceutical agents can not use paraffin sections, the general material can be produced by this method, but with longer production time, the process is complicated and the material is easy to be hard or Crisp and other shortcomings. The law and more rotary slicing machine slicing.
Paraffin preparation procedures are as follows:
(A) material collection and segmentation
Collection materials should be based on the purpose and requirements of the film, the material should be representative, no pests or other damage, such as pathological anatomy to observe, you have to take lesions, the site of the disease. The collected material should be immediay put in the specimen box or wet cloth wrapped up and back to the laboratory for segmentation and fixation (if conditions can also be collected after the trial split and fixed). In order for the fixed liquid to rapidly penetrate the material, the material should be divided. Divide action to quickly, to prevent material wilting. Split the size of the block should be small should not be large, generally not more than 1cm3, killed immediay after the split.