食物感染流感病毒診斷試劑盒（日本榮研）

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食物感染流感病毒診斷試劑盒（日本榮研）

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5、DAB顯色后發(fā)現切片著色不均勻：如一片著色，一片不著色或染色淺？
著色不均勻可能與你的實驗手法有很大關系，可能原因有：
（1）切出的片子厚度本身可能就不一樣，切出一片厚，一片薄，這樣可能造成著色深淺不一。
（2）有可能是你 顯色液底物加到片子上后沒有搖勻，造成局部底物濃度不*，所以加完顯色液后將片子來回左右晃動幾下，使片子上所有區(qū)域的底物濃度*。
（3）如果你的片子是中間的染色深，靠近邊上的淺，那有可能是你蠟圈畫的太小，顯色液覆蓋的面積不過大而產生了邊緣效應。zui外側的組織片離顯色液外緣0.5 cm。
良好的免疫組化染色切片是正確判斷染色結果的基礎和前提。由于免疫組化染色過程中存在很多步驟或環(huán)節(jié)，每一個步驟或環(huán)節(jié)都可能影響到染色的zui終結果，因此，要做好一張高質量的免疫組化切片并不是一件非常容易的事。需要病理技術員和病理醫(yī)生密切配合、相互協(xié)調、共同努力才能保證做出合格的免疫組化切片。雖然免疫組化染色可以存在各種各樣的問題，但從染色的結果看，一般可分為兩類：無色片（即無陽性信號）和“ 雜音”染色片（有陽性信號）。

5, DAB color chips and found uneven coloring: If a piece of coloring, a non-coloring or staining?
Uneven coloring may be related to your experimental approach, probably for a number of reasons:
(1) the thickness of the cut out film itself may not be the same, cut out a thick, thin, which may cause shades of color.
(2) it may be your chromogenic substrate added to the film after shaking did not shake, resulting in inconsistent local substrate concentration, so after the end of the color liquid is best to move back and forth around the film a few times so that all areas on the film The same substrate concentration.
(3) If your film is deep in the middle, near the edge of the shallow, it may be your wax ring painting is too small, the color of the liquid coverage area is too large and have an edge effect. The outermost tissue piece is best 0.5 cm from the outer edge of the color reagent.
Good immunohistochemical staining is the correct basis for judging the results of staining and the premise. Due to the many steps or steps involved in immunohistochemical staining, each step or step may affect the final result of staining. Therefore, it is not easy to make a high-quality immunohistochemical slice. Requires pathological technicians and pathologists in close cooperation, coordination and work together to ensure that qualified immunohistochemical slices. Although there are a variety of problems with immunohistochemical staining, there are generally two types of staining that result from staining: colorless (ie, no positive) and "murmur" (positive).