PCR試劑 細(xì)菌性腸胃炎的9種菌屬PCR核酸檢測(cè)試劑盒

廣州健侖生物科技有限公司

兩種準(zhǔn)備使用lyo master混合物（各8孔條），用于檢測(cè)結(jié)腸彎曲桿菌/空腸彎曲菌/紅嘴鷗彎曲桿菌，艱難梭菌，大腸桿菌Verooxin陽(yáng)性，沙門(mén)氏菌屬，志賀氏菌屬，腸侵襲性大腸桿菌，小腸結(jié)腸炎耶爾森氏菌包括內(nèi)部對(duì)照。
Two ready to use lyo master mixes (8-well strips each) for detection of Campylobacter coli/jejuni /lari, Clostridium difficile, Escherichia coli verotoxin positives, Salmonella spp., Shigella spp., enteroinvasive Escherichia coli, Yersinia enterocolitica and internal control

PCR試劑 細(xì)菌性腸胃炎的9種菌屬PCR核酸檢測(cè)試劑盒

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二、消除非特異性染色的方法 
消除熒光抗體非特異性染色的方法應(yīng)根據(jù)產(chǎn)生的原因采取適當(dāng)?shù)姆椒?，常用的方法有以下幾種： 
（一）動(dòng)物臟器粉末吸收法 
常用肝粉（豬、大白鼠或小白鼠），其次是骨髓粉、鼠腦粉和雞胚粉等。每毫升熒光抗體中加入肝粉50～100mg,在離心管中充分混勻，在室溫中振動(dòng)2h，4℃中過(guò)夜，再攪拌10min，高速離心（3000～15000r/min）30min，1～2次后，即可使用其上清液。吸收一般應(yīng)在臨用前進(jìn)行，吸收后之熒光抗體保存冰箱中勿超過(guò)2周。染色應(yīng)作吸收前后之比較，吸收時(shí)可先用緩沖鹽水將組織干粉浸濕，離心（3000～15000r/min）30min，除去上清液，再加入熒光抗體進(jìn)行吸收，以免消耗過(guò)多的抗體。 
肝粉或新鮮細(xì)胞吸收是一種非特異性的消除方法，對(duì)熒光抗體的熒光色素和蛋白都有吸附作用。如檢查組織中的病毒抗原時(shí)，也可用相同的組織干粉或勻漿沉淀物吸收之。 
用臟器肝粉吸收對(duì)熒光抗體損失較多，如果根據(jù)Hiramotos氏等的方法將組織的20%生理鹽水勻漿液，用生理鹽水洗2～3次，12000r/min  10min離心沉淀，用其沉淀物吸收其熒光抗體即能*達(dá)到目的，京極方久氏認(rèn)為這樣吸收對(duì)熒光抗體幾乎沒(méi)有損失，他們常用此法，效果甚佳，吸收后放置一周左右，用時(shí)有必要再吸收一次。 

Second, to eliminate non-specific staining methods
Elimination of non-specific fluorescent antibody staining method should be based on the reasons for taking the appropriate method, commonly used methods are the following:
(A) animal organ powder absorption method
Commonly used liver powder (pigs, rats or mice), followed by bone marrow powder, rat brain and chicken embryo powder and so on. Add 50 ~ 100mg of liver powder per ml of fluorescent antibody, mix thoroughly in a centrifuge tube, shake at room temperature for 2h, overnight at 4 ℃, stir for 10min, centrifuge at high speed (3000 ~ 15000r / min) for 30min, After that, you can use the supernatant. Absorption generally should be carried out immediay before use, after the absorption of fluorescent antibodies stored in the refrigerator for not more than 2 weeks. Dyeing should be compared before and after absorption, the absorption can be washed with saline buffer wet tissue, centrifugation (3000 ~ 15000r / min) 30min, remove the supernatant, then add fluorescent antibodies to absorb, so as not to consume too much antibody.
Liver powder or fresh cell absorption is a nonspecific method of elimination, the fluorescence of fluorescent antibodies and proteins have adsorption. If you check the virus antigen in your tissue, you can also use the same tissue dry powder or homogenate sediment absorption.
With the organism liver powder absorption of fluorescent antibody loss more, according to Hiramotos's method of tissue 20% normal saline homogenate, washed with saline 2-3 times, 12000r / min 10min centrifugal precipitation, with its precipitate Absorption of its fluorescent antibodies that can fully achieve the purpose, Kyrgyzstan side Kyrgyzstan think this absorption of almost no loss of fluorescent antibodies, they commonly used this method, the effect is very good, after absorption for a week or so, when necessary it is necessary to absorb again.